Simultaneous Identification of Multiple β-Lactamases in Acinetobacter baumannii in Relation to Carbapenem and Ceftazidime Resistance, Using Liquid Chromatography-Tandem Mass Spectrometry.

نویسندگان

  • Hein Trip
  • Katrin Mende
  • Joanna A Majchrzykiewicz-Koehorst
  • Norbert J A Sedee
  • Albert G Hulst
  • Hugo-Jan Jansen
  • Clinton K Murray
  • Armand Paauw
چکیده

Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinical Acinetobacter baumannii isolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated an added value for LC-MS/MS in antimicrobial susceptibility testing.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 53 6  شماره 

صفحات  -

تاریخ انتشار 2015